IMAGING ELECTROPHORETIC GELS WITH A SCANNING BEAM LASER MACROSCOPE

被引：4

作者：

SETO, EK

DAMASKINOS, S

DIXON, AE

DIEHLJONES, WL

MANDATO, CA

机构：

[1] UNIV WATERLOO,DEPT PHYS,WATERLOO,ON N2L 3G1,CANADA

[2] UNIV WATERLOO,DEPT BIOL,WATERLOO,ON N2L 3G1,CANADA

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 06期

关键词：

MACROSCOPE; GEL SCANNER; DNA GEL; PROTEIN GEL; F-THETA LASER SCAN LENS;

D O I：

10.1002/elps.11501601157

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A scanning beam laser macroscope* has been developed which scans an area of 7.5 X 7.5 cm in 5 s. This new imaging system is examined as a potential tool for scanning electrophoretic gels. A specially-designed telecentric, f*Theta laser scan lens is used in the instrument to achieve a linear scan and a flat focal plane. The laser scan lens focuses the incoming beam from a laser to a 10 mu m spot inside the gel. A raster scan is performed across the gel and the signal is detected with a photomultiplier, forming a 512 X 512 digital image stored as a computer file. Silver-stained protein polyacrylamide gels have been imaged in transmission and double-transmission, while DNA agarose gels (stained with ethidium bromide) have been imaged in fluorescence with better than 25 pg sensitivity. The macroscope has the advantage that it is not tied to the electrophoresis system as are end-of-line scanners, and the scan is rapid, so that several gels can be scanned in a very short time.

引用

页码：934 / 940

页数：7

共 10 条

[1]

Sutherland J.C., Adv. Electrophoresis, 6, pp. 1-42, (1993)

[2]

Sutherland J.C., Lin B., Monteleone D.C., Mugavero J., Sutherland B.M., Trunk J., Anal. Biochem., 163, pp. 446-457, (1987)

[3]

Quesada M.A., Rye H.S., Gingrich J.C., Glazer A.N., Mathies R.A., BioTechniques, 10, pp. 616-625, (1991)

[4]

Mathies R.A., Scherer J.R., Quesada M.A., Rye H.S., Glazer A.N., Rev. Sci. Instrum., 65, pp. 807-812, (1994)

[5]

Dixon A.E., Damaskinos S., Ribes A., Beesley K.M., (1995)

[6]

Ishino Y., Mineno J., Inoue T., Fujimiya H., Yamamoto K., Tamura T., Homma M., Tanaka K., Kato I., BioTechniques, 13, pp. 936-943, (1992)

[7]

Beesley K.M., Damaskinos S., Dixon A.E., J. Forensic Sci., 40, pp. 10-17, (1995)

[8]

Glazer A.N., Peck K., Mathies R.A., Proc. Natl. Acad. Sci. USA, 87, pp. 3851-3855, (1990)

[9]

Rye H.S., Quesada M.A., Peck K., Mathies R.A., Glazer A.N., Nucleic Acids Res., 19, pp. 327-333, (1991)

[10]

Rye H.S., Yue S., Quesada M.A., Haugland R.P., Mathies R.A., Glazer A.N., Methods Enzymol., 217, pp. 414-431, (1993)

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