STATISTICAL ANALYSIS OF DATA FROM COUNTERCURRENT DISTRIBUTION .3. BILIARY AND URINARY EXCRETION PATTERNS OF ESTRIOL IN A HUMAN SUBJECT

The application of the previously reported (1,2), new mathematical approach for the analysis in countercurrent distribution data, to the determination of the percentage and number of compounds in the whole CCD train rather than to individual peaks is found to be very successful. The utility of the method is typically examplified by the analysis of biliary and urinary excretion patterns of estriol-6, 7-H3 in a human subject. The fractionation of these biological media was also performed by CCD, but without prior treatment of any type. This assured both minimal artifact formation and no loss of radioactivity. The combination of mathematical approach and CCD therefore provides a truer picture of the excretion pattern than has been hitherto possible. © 1968.