CELL SPECIFIC DISTRIBUTION OF GUANINE NUCLEOTIDE-BINDING REGULATORY PROTEINS IN RAT PITUITARY-TUMOR CELL-LINES

To investigate the effects of guanine nucleotide-binding regulatory proteins (G proteins) on hormonal regulation of prolactin (PRL) synthesis and secretion, the qualitative distribution of G protein alpha-subunits and their mRNAs was studied in three functionally different pituitary tumour cell lines (GH cells) and normal rat pituitary tissue. Levels of basal and modulated adenylyl cyclase (AC) and phospholipase C (PLC) activities are also included. GH cells and pituitary tissue contained various amounts of mRNAs and protein for G(s)-alpha, G(i-2)-alpha, G(i-3)-alpha and G(o)-alpha, while mRNA for G(i-1)-alpha was only detected in normal pituitary tissue. G(z)-alpha/G(x)-alpha mRNA was expressed in all pituitary cell lines as well as in pituitary tissue. G(o)-alpha mRNA and G(z)-alpha/G(x)-alpha mRNA displayed size heterogeneity. These findings may have importance in the understanding of hormone regulation of second messenger systems.