ABOLITION OF AN INDUCIBLE HIGHLY ANIONIC PEROXIDASE-ACTIVITY IN TRANSGENIC TOMATO

被引:60
作者
SHERF, BA [1 ]
BAJAR, AM [1 ]
KOLATTUKUDY, PE [1 ]
机构
[1] OHIO STATE BIOTECHNOL CTR, 1060 CARMACK RD, COLUMBUS, OH 43210 USA
关键词
D O I
10.1104/pp.101.1.201
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Locally induced expression of a highly anionic peroxidase has previously been correlated temporally and spatially with suberization of tissues responding to pathogen assault, wounding, or exogenously applied abscisic acid or fungal elicitors. DNA sequences corresponding to the 5' regions of two tomato (Lycopersicon esculentum) genes encoding homologous anionic peroxidases were fused, inserted into a pTi-based plasmid designed to express a composite antisense transcript, and introduced into tomato via Agrobacterium-mediated transformation. RNA gel-blot analyses showed high expression of the antisense transcript in most transgenic plants and no detectable induction of native anionic peroxidase transcripts in wounded or abscisic acid- or pathogen-treated tissues. Plants and fruits expressing the antisense transcript appeared normal in all respects. Electrophoretic analysis of anionic proteins from selected transgenic plants showed no detectable anionic peroxidase protein or activity. Depolymerization of polymeric material from the wound periderm of transgenic tomato fruits and analysis of the aliphatic products by gas-liquid chromatography/mass spectrometry showed that the content and composition of C-16/C18 omega-hydroxy and dicarboxylic acids, characteristic of suberin, were not affected by the absence of the anionic peroxidase. Autofluorescence generated from cell wall phenolics at the wound lesion was also not affected by the absence of the highly anionic peroxidase.
引用
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页码:201 / 208
页数:8
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