Non-Destructive RAPD Genetic Diagnostics of Microspore-Derived Brassica Embryos

被引：37

作者：

Horn, Peggy ^{[2
]}

Rafalski, Antoni ^{[1
]}

机构：

[1] Dupont Co, Expt Stn, Agr Prod Biotechnol, Wilmington, DE 19880 USA

[2] Univ Canberra, Belconnen, ACT 2616, Australia

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1992年 / 10卷 / 03期

关键词：

Genetic mapping; RAPD; random primers; Brassica; microspore-derived embryo;

D O I：

10.1007/BF02668361

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate the application of the RAPD genetic polymorphism assay (Williams et al., 1990, Welsh and McClelland 1990), to the determination of the genotype of immature, microspore - derived haploid embryos of Brassica napus. Several hundred assays can be performed on the DNA obtained from a cotyledon fragment, and the remaining embryo can be regenerated. Thus, the assay can be used for "prenatal" diagnostics of embryo-regenerated plants, and can facilitate selection of defined genotypes in plant breeding. A suitable population of embryos could also be used for the construction of RAPD genetic maps.

引用

页码：285 / 293

页数：9

共 5 条

[1] SEGREGATION OF RANDOM AMPLIFIED DNA MARKERS IN F1 PROGENY OF CONIFERS [J].

CARLSON, JE ;

TULSIERAM, LK ;

GLAUBITZ, JC ;

LUK, VWK ;

KAUFFELDT, C ;

RUTLEDGE, R .

THEORETICAL AND APPLIED GENETICS, 1991, 83 (02) :194-200

[2]

DELLAPORTA SL, 1985, MOL BIOL PLANTS LAB, P36

[3] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS [J].

GAMBORG, OL ;

MILLER, RA ;

OJIMA, K .

EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) :151-+

[4] ANTHER CULTURE OF BRASSICA-NAPUS IN A LIQUID CULTURE-MEDIUM [J].

LICHTER, R .

ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE, 1981, 103 (03) :229-237

[5]

Maniatis T., 1982, MOL CLONING LAB MANU

← 1 →