DETERMINATION OF INFLAMMATORY BOWEL-DISEASE ACTIVITY BY BREATH PENTANE ANALYSIS

被引:60
作者
KOKOSZKA, J
NELSON, RL
SWEDLER, WI
SKOSEY, J
ABCARIAN, H
机构
[1] UNIV ILLINOIS,COLL MED,DEPT SURG,CHICAGO,IL 60680
[2] UNIV ILLINOIS,COLL MED,DEPT RHEUMATOL,CHICAGO,IL 60680
关键词
INFLAMMATORY BOWEL DISEASE; PENTANE; LIPID PEROXIDATION; OXYGEN RADICALS; INDIUM-111-LABELED GRANULOCYTE SCAN;
D O I
10.1007/BF02049868
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
PURPOSE: Quantitative determination of breath pentane, an alkane generated by peroxidation of cellular fatty acids, has been used as a noninvasive determinant of inflammation. Herein we report the first examination of the relationship between breath pentane and intestinal inflammation in humans. METHODS: Patients (N = 33), either with a known history of inflammatory bowel disease (IBD) with symptoms of relapse or with no known history of but having symptoms consistent with IBD, were evaluated with indium- 111 -labeled leukocyte imaging to assess the presence of active inflammation. At the time of the indium scan, the exhaled breath of the patients was obtained via a collecting tube. Gas chromatography was used to quantify the pentane content. and these values were compared with graded indium scans. RESULTS: The range of breath pentane found in our population (36 determinations in 33 patients) was from 0 to 38.4 nmol/l of exhaled air. For patients with negative scans, the mean pentane was 2.1 nmol/l, for intermediate scans 3.1, for positive scans 4.3, and for nonintestinal nuclide imaging 5.5 [P = 0.005 by analysis of variance (ANOVA)]. CONCLUSIONS: We have previously demonstrated the correlation of breath pentane with gross and histologic evidence of intestinal inflammation in a rodent colitis model. This current study also demonstrates that pentane analysis can be correlated with inflammatory bowel disease activity in humans.
引用
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页码:597 / 601
页数:5
相关论文
共 14 条
[1]   BIOLOGICAL DEFENSE MECHANISMS - PRODUCTION BY LEUKOCYTES OF SUPEROXIDE A POTENTIAL BACTERICIDAL AGENT [J].
BABIOR, BM ;
KIPNES, RS ;
CURNUTTE, JT .
JOURNAL OF CLINICAL INVESTIGATION, 1973, 52 (03) :741-744
[2]   THE ROLE OF IN-111 WHITE BLOOD-CELLS IN INFLAMMATORY BOWEL-DISEASE [J].
FROELICH, JW ;
FIELD, SA .
SEMINARS IN NUCLEAR MEDICINE, 1988, 18 (04) :300-307
[3]   BREATH PENTANE EXCRETION AS A MARKER OF DISEASE-ACTIVITY IN RHEUMATOID-ARTHRITIS [J].
HUMAD, S ;
ZARLING, E ;
CLAPPER, M ;
SKOSEY, JL .
FREE RADICAL RESEARCH COMMUNICATIONS, 1988, 5 (02) :101-106
[4]   SIMPLE METHOD OF MEASURING BREATH HYDROGEN IN CARBOHYDRATE MALABSORPTION BY END-EXPIRATORY SAMPLING [J].
METZ, G ;
GASSULL, MA ;
LEEDS, AR ;
BLENDIS, LM ;
JENKINS, DJA .
CLINICAL SCIENCE AND MOLECULAR MEDICINE, 1976, 50 (03) :237-240
[5]   INDIUM-111-LABELED GRANULOCYTE SCAN IN THE DIAGNOSIS AND MANAGEMENT OF ACUTE INFLAMMATORY BOWEL-DISEASE [J].
NELSON, RL ;
SUBRAMANIAN, K ;
GASPARAITIS, A ;
ABCARIAN, H ;
PAVEL, DG .
DISEASES OF THE COLON & RECTUM, 1990, 33 (06) :451-457
[6]   QUANTITATIVE-DETERMINATION OF PENTANE IN EXHALED AIR CORRELATES WITH COLONIC INFLAMMATION IN THE RAT COLITIS MODEL [J].
ONDRULA, D ;
NELSON, RL ;
ANDRIANOPOULOS, G ;
SCHWARTZ, D ;
ABCARIAN, H ;
BIRNBAUM, A ;
SKOSEY, J .
DISEASES OF THE COLON & RECTUM, 1993, 36 (05) :457-462
[7]   CAN IN-111 AUTOLOGOUS MIXED LEUKOCYTE SCANNING ACCURATELY ASSESS DISEASE EXTENT AND ACTIVITY IN CROHNS-DISEASE [J].
PARK, RHR ;
MCKILLOP, JH ;
DUNCAN, A ;
MACKENZIE, JF ;
RUSSELL, RI .
GUT, 1988, 29 (06) :821-825
[8]   PENTANE MEASUREMENT IN MAN AS AN INDEX OF LIPOPEROXIDATION [J].
PINCEMAIL, J ;
DEBY, C ;
DETHIER, A ;
BERTRAND, Y ;
LISMONDE, M ;
LAMY, M .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1987, 18 (1-3) :117-125
[9]  
POITRAS P, 1987, J CLIN GASTROENTEROL, V4, P418
[10]   ETHANE EVOLUTION - NEW INDEX OF LIPID PEROXIDATION [J].
RIELY, CA ;
COHEN, G ;
LIEBERMAN, M .
SCIENCE, 1974, 183 (4121) :208-210