EXPRESSION OF A SINGLE-CHAIN FV ANTIBODY AGAINST ABSCISIC-ACID CREATES A WILTY PHENOTYPE IN TRANSGENIC TOBACCO

被引:129
作者
ARTSAENKO, O
PEISKER, M
ZURNIEDEN, U
FIEDLER, U
WEILER, EW
MUNTZ, K
CONRAD, U
机构
[1] INST PFLANZENGENET & KULTURPFLANZENFORSCH, D-06466 GATERSLEBEN, GERMANY
[2] INST PFLANZENBIOCHEM, D-06120 HALLE, GERMANY
[3] RUHR UNIV BOCHUM, D-44801 BOCHUM, GERMANY
关键词
D O I
10.1046/j.1365-313X.1995.08050745.x
中图分类号
Q94 [植物学];
学科分类号
071001 [植物学];
摘要
The plant hormone abscisic acid (ABA) participates in the control of several important physiological processes in plants such as stomata regulation, seed dormancy and stress tolerance. A new strategy was developed to study these phenomena by blocking abscisic acid with intracellularly expressed specific single-chain variable fragment (scFv) antibodies. Here evidence is presented that the expression of single-chain Fv antibodies against abscisic acid in the endoplasmic reticulum of transgenic tobacco cells leads to a witty phenotype. Stomatal conductance is increased at high CO2 concentrations dependent on the level of antibody expression in leaves. Symptoms of abscisic acid deficiency were generated in the transformants although they have even higher levels of abscisic acid than wild-type plants.
引用
收藏
页码:745 / 750
页数:6
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