SOLID-PHASE EXTRACTION OF NICOTINE AND ITS METABOLITES FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION IN URINE

被引：28

作者：

ZUCCARO, P

ALTIERI, I

ROSA, M

PASSA, AR

PICHINI, S

PACIFICI, R

机构：

[1] Istituto Superiore di Sanità, 00161 Roma

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 668卷 / 01期

关键词：

D O I：

10.1016/0378-4347(95)00066-R

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A solid-phase extraction, using Extrelut-1 glass columns, has been applied to urine samples of both passive and active smokers for high-performance liquid chromatographic determination of nicotine and its metabolites cotinine and trans-3'-hydroxycotinine. Chromatography was performed using a reversed-phase LC(8)DB column and a mobile phase consisting of water-acetonitrile (80:9, v/v) containing 5 ml triethylamine, 670 mg/l sodium heptanesulphonate, and 0.034 M each of K2HPO4 and citric acid (pH 4.4), at a flow-rate of 1.6 ml/min. The results obtained indicate that solid-phase extraction is a reliable and quick procedure which can be applied also to other nicotine metabolites.

引用

页码：187 / 188

页数：2