PROTEIN-CONTENT AND ENZYME-ACTIVITIES IN METHANOL-GROWN AND ACETATE-GROWN METHANOSARCINA-THERMOPHILA

被引:37
作者
JABLONSKI, PE
DIMARCO, AA
BOBIK, TA
CABELL, MC
FERRY, JG
机构
[1] VIRGINIA POLYTECH INST & STATE UNIV,DEPT ANAEROB MICROBIOL,BLACKSBURG,VA 24061
[2] UNIV ILLINOIS,DEPT MICROBIOL,URBANA,IL 61801
关键词
D O I
10.1128/jb.172.3.1271-1275.1990
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The cell extract protein content of acetate- and methanol-grown Methanosarcina thermophila TM-1 was examined by two-dimensional polyacrylamide gel electrophoresis. More than 100 mutually exclusive spots were present in acetate- and methanol-grown cells. Spots corresponding to acetate kinase, phosphotransacetylase, and the five subunits of the carbon monoxide dehydrogenase complex were identified in acetate-grown cells. Activities of formylmethanofuran dehydrogenase, formylmethanofuran:tetrahydromethanopterin formyltransferase, 5,10-methenyltetrahydromethanopterin cyclohydrolase, methylene tetrahydromethanopterin:coenzyme F420 oxidoreductase, formate dehydrogenase, and carbonic anhydrase were examined in acetate- and methanol-grown Methanosarcina thermophila. Levels of formyltransferase in either acetate- or methanol-grown Methanosarcina thermophila were approximately half the levels detected in H2-CO2-grown Methanobacterium thermoautotrophicum. All other enzyme activities were significantly lower in acetate- and methanol-grown Methanosarcina thermophila.
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页码:1271 / 1275
页数:5
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