IDENTIFICATION OF INDIVIDUAL (1-]3,1-]4)-BETA-D-GLUCANASE ISOENZYMES IN EXTRACTS OF GERMINATED BARLEY USING SPECIFIC MONOCLONAL-ANTIBODIES

被引：15

作者：

HOJ, PB ^{[1
]}

HOOGENRAAD, NJ ^{[1
]}

HARTMAN, DJ ^{[1
]}

YANNAKENA, H ^{[1
]}

FINCHER, GB ^{[1
]}

机构：

[1] LA TROBE UNIV, DEPT BIOCHEM, COMMONWEALTH RES CTR PROT & ENZYME TECHNOL, BUNDOORA, VIC 3083, AUSTRALIA

来源：

JOURNAL OF CEREAL SCIENCE | 1990年 / 11卷 / 03期

关键词：

EDTA; ELISA; enzyme-linked immunosorbent assay; ethylenediamintetraacetate; SDS-PAGE; sodium dodecyl sulphate polyacrylamide gel electrophoresis;

D O I：

10.1016/S0733-5210(09)80170-7

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Specific monoclonal antibodies have been used to distinguish between individual barley (1 → 3,1 → 4)-β-glucan endohydrolase (EC 3.2.1.73) isoenzymes-in enzyme linked immunosorbent (ELISA) and Western transfer assays. In addition, a monoclonal antibody that recognises both isoenzymes has been isolated. The antibodies, which are of the immunoglobulin IgG1 subclass and carry kappa light chains, do not cross-react with barley (1 → 3)-β-glucanases or with other proteins found in water-soluble extracts of germinated barley grain. The potential applications of these specific antibodies in barley breeding programmes and in physiological studies are discussed. © 1990, Academic Press Limited. All rights reserved.

引用

页码：261 / 268

页数：8

共 25 条

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