NONINVASIVE EVALUATION OF SOMATIC EMBRYOGENESIS

被引：16

作者：

HARRELL, RC ^{[1
]}

BIENIEK, M ^{[1
]}

CANTLIFFE, DJ ^{[1
]}

机构：

[1] UNIV FLORIDA,DEPT VEGETABLE CROPS,GAINESVILLE,FL 32611

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 39卷 / 04期

关键词：

CALLUS SUSPENSION CULTURES; MACHINE VISION SYSTEM; IPOMOEA-BATATAS; SOMATIC EMBRYOGENESIS;

D O I：

10.1002/bit.260390403

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Callus Suspension Cultures of Ipomea batatas Poir. cv. White Star were grown in an airlift bioreactor. A machine vision system was used to monitor nondestructively callus growth during a 10 day culture period. Growth data obtained with this system included the overall reactor population and population estimates for the 200-1200-mu-m fractions at 200-mu-m intervals. A model of callus growth was developed to explain the mechanics of callus enlargement. The model was based on the assumptions that (1) the calli could not subdivide or shrink, (2) there was a fixed percentage of the initial population for each fraction that was nonviable, and (3) growth rates did not vary with time during the culture period. It was determined that the growth rates and nonviable ratios decreased as fraction size increased.

引用

页码：378 / 383

页数：6

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