2-PARAMETER MOBILE PHASE OPTIMIZATION FOR THE SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF DOPAMINE, SEROTONIN AND RELATED-COMPOUNDS IN MICRODISSECTED RAT-BRAIN NUCLEI

被引：22

作者：

LEMA, M ^{[1
]}

OTERO, J ^{[1
]}

MARCO, J ^{[1
]}

机构：

[1] UNIV SANTIAGO,FAC BIOL,DEPT PHYSIOL,E-15706 SANTIAGO,SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 547卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88634-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new high-pressure liquid chromatography method with electrochemical detection is described that allows the simultaneous determination of dopamine, serotonin, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylacetic acid, 5-hydroxytryptophan and 5-hydroxyindoleacetic acid in microdissected nuclei from individual rat brains. No sample pre-treatment steps are required. Resolution and analysis time were optimized by a simple limited optimization procedure, involving two-parameter factorial design.

引用

页码：113 / 120

页数：8

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