AUTOMATED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF IOPENTOL IN HUMAN PLASMA AND WHOLE-BLOOD USING ONLINE DIALYSIS AS SAMPLE PREPARATION

被引：24

作者：

ANDRESEN, AT

JACOBSEN, PB

RASMUSSEN, KE

机构：

[1] NYCOMED AS,N-0401 OSLO 4,NORWAY

[2] UNIV OSLO,INST PHARM,N-0316 OSLO 3,NORWAY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 575卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80508-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fully automated high-performance liquid chromatographic method has been developed for the analysis of the radiographic contrast agent iopentol in human plasma and whole blood. This method is based on on-line sample preparation with dialysis followed by pre-column enrichment of the dialysate. The method was compared with a manual sample preparation method based on protein precipitation with tetrahydrofuran. The inter-assay and intra-assay variations and the limits of quantitation were the same for both methods. The on-line combination of dialysis and column-switching high-performance liquid chromatography was shown to be a reliable and time-saving technique for monitoring iopentol in human whole blood and plasma.

引用

页码：93 / 99

页数：7

共 13 条

[1] ONLINE COMBINATION OF DIALYSIS AND COLUMN-SWITCHING LIQUID-CHROMATOGRAPHY AS A FULLY AUTOMATED SAMPLE PREPARATION TECHNIQUE FOR BIOLOGICAL SAMPLES - DETERMINATION OF NITROFURAN RESIDUES IN EDIBLE PRODUCTS [J].