TRINUCLEOTIDE PHOSPHORAMIDITES - IDEAL REAGENTS FOR THE SYNTHESIS OF MIXED OLIGONUCLEOTIDES FOR RANDOM MUTAGENESIS

被引：165

作者：

VIRNEKAS, B ^{[1
]}

GE, LM ^{[1
]}

PLUCKTHUN, A ^{[1
]}

SCHNEIDER, KC ^{[1
]}

WELLNHOFER, G ^{[1
]}

MORONEY, SE ^{[1
]}

机构：

[1] UNIV ZURICH,INST BIOCHEM,CH-8057 ZURICH,SWITZERLAND

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 25期

关键词：

D O I：

10.1093/nar/22.25.5600

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trinucleotide phosphoramidites representing codons for all 20 amino acids have been prepared and used in automated, solid-phase DNA synthesis. In contrast to an earlier report, we show that these substances can be used to introduce entire codons into oligonucleotides in excess of 98% yield, and are ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.

引用

页码：5600 / 5607

页数：8

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