MESSENGER-RNA EXPRESSION OF GROWTH-FACTORS IN GLOMERULI FROM DIABETIC RATS

被引：325

作者：

NAKAMURA, T ^{[1
]}

FUKUI, M ^{[1
]}

EBIHARA, I ^{[1
]}

OSADA, S ^{[1
]}

NAGAOKA, I ^{[1
]}

TOMINO, Y ^{[1
]}

KOIDE, H ^{[1
]}

机构：

[1] JUNTENDO UNIV, SCH MED, DEPT MED, DIV NEPHROL, BUNKYO KU, TOKYO 113, JAPAN

来源：

DIABETES | 1993年 / 42卷 / 03期

关键词：

D O I：

10.2337/diabetes.42.3.450

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Evaluations of glomerular mRNA levels encoding for PCNA, TNF-alpha, PDGF-A and -B chains, TGF-beta, IGF-I, bFGF, and EGF were made at 4, 12, and 24 wk after injection of STZ in Sprague-Dawley rats. The mRNA levels for PCNA, TNF-alpha, PDGF-B chain, TGF-beta, and bFGF increased with age in STZ-induced diabetic rats. At 24 wk after STZ injection, mRNA levels for PCNA, TNF-alpha, PDGF-B chain, TGF-beta, and bFGF were increased 3.8-fold, (P < 0.01), 4.2-fold (P < 0.01), 4.0-fold (P < 0.01), 5.2-fold (P < 0.001), and 3.6-fold (P < 0.01), respectively, in the glomeruli of diabetic rats when compared with control rats. In contrast, mRNA levels for IGF-I, PDGF-A chain, and EGF were not altered in glomeruli from diabetic and control rats throughout the experimental period. Insulin treatment partially ameliorated the increase in mRNA levels for PCNA, TNF-alpha, PDGF-B chain, TGF-beta, and bFGF in the glomeruli of diabetic rats. These data indicate that alterations in growth factor mRNA levels in glomeruli may be a manifestation of diabetic nephropathy, and that hyperglycemia or insulin deficiency may play a role in abnormal growth factor gene regulation.

引用

页码：450 / 456

页数：7

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