ARTIFACT ASSOCIATED WITH ELUTION OF STEROID AFFINITY RESINS BY HIGH-CONCENTRATIONS OF LIGANDS

At high (μM) concentrations and in the absence of protein, [H]-progesterone, [3H]-oestradiol and [3H]-diethylstilboestrol form aggregates which elute in the void volume of Sephadex G-25 and G-75 columns. This could be a potential source of error in calculating the amount of receptor actually present in the eluate from an affinity column. © 1979.