BIOLOGICAL X-RAY-MICROANALYSIS

被引：182

作者：

HALL, TA

机构：

[1] Biological Microprobe Laboratory, Department of Zoology, University of Cambridge

来源：

JOURNAL OF MICROSCOPY | 1979年 / 117卷 / SEP期

关键词：

D O I：

10.1111/j.1365-2818.1979.tb00236.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

By means of X‐ray microanalysis it is now practical to detect approximately 10−19 g of an element in a static‐probe analysis within an ultrathin section, with analytical spatial resolution in the range 20–30 nm. The main difficulties for biological microanalysis are connected not with sensitivity but with specimen preparation and beam damage. Careful cryopreparation, beginning with the quench‐freezing of a small block of tissue, is essential even for determining the storage sites, or sites of binding in vivo, of physiologically active elements. In frozen‐dried or frozen‐hydrated sections of quench‐frozen tissue, it is now possible to measure local mass fractions of diffusible as well as of bound elements. 1979 Blackwell Science Ltd

引用

页码：145 / 163

页数：19

共 127 条

[1]

ANDERSEN C. A., 1967, METHODS BIOCHEM ANAL, V15, P147, DOI 10.1002/9780470110331.ch4

[2] QUALITY OF X-RAY MICROANALYSIS IN ULTRA-SOFT X-RAY REGION [J].