DIAPAUSING PUPAE OF HYALOPHORA-CECROPIA - AN ALTERNATIVE HOST FOR BACULOVIRUS MEDIATED EXPRESSION

被引：4

作者：

HELLERS, M ^{[1
]}

STEINER, H ^{[1
]}

机构：

[1] UNIV STOCKHOLM,DEPT MICROBIOL,S-10691 STOCKHOLM,SWEDEN

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1992年 / 22卷 / 01期

关键词：

AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS; BACULOVIRUS; HYALOPHORA-CECROPIA; PROTEIN EXPRESSION; TRICHOPLUSIA-NI;

D O I：

10.1016/0965-1748(92)90097-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The insect baculovirus Autographa californica nuclear polyhedrosis virus was found to propagate in the North American silk moth Hyalophora cecropia after injection. Wild type virus and four recombinant viruses were used to infect pupae of H. cecropia, larvae of Trichoplusia ni and Spodoptera frugiperda cells in culture. The expression of recombinant proteins were compared in the different hosts and it was found that H. cecropia is equal to or in some cases better than T. ni as a host for heterologous expression in vivo. The expression of recombinant proteins in H. cecropia also has several practical advantages to that of expression in T. ni.

引用

页码：35 / 39

页数：5

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