CLONING AND SEQUENCE-ANALYSIS OF A CANDIDA-MALTOSA GENE WHICH CONFERS RESISTANCE TO CYCLOHEXIMIDE

被引：38

作者：

SASNAUSKAS, K

JOMANTIENE, R

LEBEDIENE, E

LEBEDYS, J

JANUSKA, A

JANULAITIS, A

机构：

[1] Department of Molecular Biology, Institute of Applied Enzymology

来源：

GENE | 1992年 / 116卷 / 01期

关键词：

RECOMBINANT DNA; EXPRESSION; SACCHAROMYCES-CEREVISIAE; NUCLEOTIDE SEQUENCE;

D O I：

10.1016/0378-1119(92)90636-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A CYH(R) gene from Candida maltosa, which confers resistance to cycloheximide, was cloned in Saccharomyces cerevisiae. A 2.3-kb DNA fragment carrying this gene was sequenced, and an open reading frame able to encode 553 amino acids (aa) was found in the sequence. Computer searches of the GenBank, EMBL, SWIS-PROT and Gen-Pept databases using the FASTA program failed to detect any proteins with extensive similarities to the deduced aa sequence for CYH(R). The cloned gene transforms S. cerevisiae at a frequency similar to auxotrophic markers and can be used as a dominant selectable marker for introducing recombinant plasmids into wild-type strains of S. cerevisiae, as well as for gene disruption experiments.

引用

页码：105 / 108

页数：4

共 13 条

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