ENHANCEMENT OF SELF-ASSOCIATION OF HUMAN SPECTRIN BY POLYETHYLENE-GLYCOL

被引：21

作者：

COLE, N ^{[1
]}

RALSTON, GB ^{[1
]}

机构：

[1] UNIV SYDNEY,DEPT BIOCHEM,SYDNEY,NSW 2006,AUSTRALIA

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1994年 / 26卷 / 06期

基金：

澳大利亚研究理事会;

关键词：

SPECTRIN; POLYETHYLENE GLYCOL; SELF-ASSOCIATION; ELECTROPHORESIS; EXCLUDED VOLUME; CROWDING;

D O I：

10.1016/0020-711X(94)90109-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. In the presence of polyethylene glycol, the self-association of human spectrin is enhanced in a manner that depends approx. exponentially on the-mass concentration-of polyethylene glycol. 2. For a given mass concentration, the enhancement is independent of the molecular weight of the polyethylene glycol. 3. These data are consistent with the operation of excluded volume effects, and support the contention that the association of spectrin is likely to be increased in the presence of the high concentration of hemoglobin within the erythrocyte in vivo.

引用

页码：799 / 804

页数：6

共 20 条

[1] PROTEIN PARTITIONING IN 2-PHASE AQUEOUS POLYMER SYSTEMS .2. ON THE FREE-ENERGY OF MIXING GLOBULAR COLLOIDS AND FLEXIBLE POLYMERS [J].