CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES ON CYTOSOLIC (CLASS-1) ALDEHYDE DEHYDROGENASE FROM SHEEP LIVER

被引：6

作者：

BAKER, HM ^{[1
]}

BROWN, RL ^{[1
]}

DOBBS, AJ ^{[1
]}

BLACKWELL, LF ^{[1
]}

BUCKLEY, PD ^{[1
]}

HARDMAN, MJ ^{[1
]}

HILL, JP ^{[1
]}

KITSON, KE ^{[1
]}

KITSON, TM ^{[1
]}

BAKER, EN ^{[1
]}

机构：

[1] MASSEY UNIV,DEPT CHEM & BIOCHEM,PALMERSTON NORTH,NEW ZEALAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 241卷 / 02期

关键词：

CRYSTALLIZATION; ALDEHYDE DEHYDROGENASE; X-RAY DIFFRACTION;

D O I：

10.1006/jmbi.1994.1494

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytosolic (Class 1) aldehyde dehydrogenase (AIDH) from sheep liver has been crystallized in a form suitable for X-ray diffraction studies. The crystals, grown by vapour diffusion using 6.5 to 7.5% methoxypolyethylene glycol 5000 as precipitant, at pH 6.5, are orthorhombic with cell dimensions a = 80.7, b = 92.5, c = 151.6 a, space-group P2(1)2(1)2(1), and one dimer in the asymmetric unit;. The crystals diffract to at least 2.8 Angstrom resolution. Although unmodified AIDH crystallized readily, a key factor in obtaining diffraction-quality crystals was the covalent attachment of an active site reporter group, provided by 3,4-dihydro-3-methyl-6-nitro-2H-1,3-benzoxazin-2-one.

引用

页码：263 / 264

页数：2

共 11 条

[1] THIOL PROTEASES AND ALDEHYDE DEHYDROGENASES - EVOLUTION FROM A COMMON THIOLESTERASE PRECURSOR
HEMPEL, J
NICHOLAS, H
JORNVALL, H
[J]. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 1991, 11 (03) : 176 - 183
[2] CRYSTALLIZATION AND PRELIMINARY-X-RAY INVESTIGATION OF BOVINE LIVER MITOCHONDRIAL ALDEHYDE DEHYDROGENASE
HURLEY, TD
WEINER, H
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1992, 227 (04) : 1255 - 1257
[3] CLONING AND COMPLETE NUCLEOTIDE-SEQUENCE OF A FULL-LENGTH CDNA-ENCODING A CATALYTICALLY FUNCTIONAL TUMOR-ASSOCIATED ALDEHYDE DEHYDROGENASE
JONES, DE
BRENNAN, MD
HEMPEL, J
LINDAHL, R
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (06) : 1782 - 1786
[4] KINGSTON RL, 1994, IN PRESS ACTA CRYS D
[5] PROBING THE ACTIVE-SITE OF CYTOPLASMIC ALDEHYDE DEHYDROGENASE WITH A CHROMOPHORIC REPORTER GROUP
KITSON, TM
KITSON, KE
[J]. BIOCHEMICAL JOURNAL, 1994, 300 : 25 - 30
[6] 3,4-DIHYDRO-3-METHYL-6-NITRO-2H-1,3-BENZOXAZIN-2-ONE, A REAGENT FOR LABELING P-NITROPHENYL ESTERASES WITH A CHROMOPHORIC REPORTER GROUP - SYNTHESIS AND REACTION WITH CHYMOTRYPSIN
KITSON, TM
FREEMAN, GH
[J]. BIOORGANIC CHEMISTRY, 1993, 21 (03) : 354 - 365
[7] LINDROTH RL, 1991, INSECT PLANT INTERAC, V3, P1
[8] SOLVENT CONTENT OF PROTEIN CRYSTALS
MATTHEWS, BW
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) : 491 - +
[9] McPherson A., 1982, PREPARATION ANAL PRO
[10] PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF CLASS-3 RAT-LIVER ALDEHYDE DEHYDROGENASE
ROSE, JP
HEMPEL, J
KUO, I
LINDAHL, R
WANG, BC
[J]. PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1990, 8 (04): : 305 - 308

← 1 2 →