HYALURONIC-ACID-DEFICIENT EXTRACELLULAR-MATRIX INDUCED BY ADDITION OF 4-METHYLUMBELLIFERONE TO THE MEDIUM OF CULTURED HUMAN SKIN FIBROBLASTS

被引：83

作者：

NAKAMURA, T ^{[1
]}

TAKAGAKI, K ^{[1
]}

SHIBATA, S ^{[1
]}

TANAKA, K ^{[1
]}

HIGUCHI, T ^{[1
]}

ENDO, M ^{[1
]}

机构：

[1] HIROSAKI UNIV, SCH MED, DEPT BIOCHEM, HIROSAKI, AOMORI 036, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 208卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1362

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of xylosyl-beta-D-(4-methylumbelliferone) and its aglycone, 4-methylumbelliferone, on hyaluronic acid synthesis were investigated in cultured human skin fibroblasts. Xylosyl-beta-D-(4-methylumbelliferone) added to the medium of cultured cell reduced the synthesis of hyaluronic acid. Furthermore, 4-methylumbelliferone reduced the production of hyaluronic acid markedly. In addition, 4-methylumbelliferone had hardly any effect on proteoglycan synthesis, whereas xylosyl-beta-D-(4-methylumbelliferone) produced a large amount of glycosaminoglycan chains. The present results indicate that cells cultured with 4-methylumbelliferone produce a hyaluronic-acid-deficient extracellular matrix, which will be useful for functional studies of hyaluronic acid. (C) 1995 Academic Press, Inc.

引用

页码：470 / 475

页数：6

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