SELECTIVE CHEMICAL AUTOLIGATION ON A DOUBLE-STRANDED DNA-TEMPLATE

被引：26

作者：

HERRLEIN, MK ^{[1
]}

LETSINGER, RL ^{[1
]}

机构：

[1] NORTHWESTERN UNIV,DEPT CHEM,EVANSTON,IL 60208

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 23期

关键词：

D O I：

10.1093/nar/22.23.5076

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that a double-stranded DNA segment serves as an effective template for spontaneously coupling short pyrimidine oligonucleotides containing terminal -P(O)(O-S- and BrCH2C(O)NH- groups. The efficiency of this autoligation depends markedly on proper base-pairing between the probe oligomers and the double-stranded target. This chemistry should be useful in designing highly selective probes for double-stranded polynucleotide segments.

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页码：5076 / 5078

页数：3

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