ESTIMATION OF FREE DOPAMINE IN THE CYTOPLASM OF THE GIANT DOPAMINE CELL OF PLANORBIS-CORNEUS BY VOLTAMMETRY AND CAPILLARY ELECTROPHORESIS

被引：139

作者：

CHIEN, JB ^{[1
]}

WALLINGFORD, RA ^{[1
]}

EWING, AG ^{[1
]}

机构：

[1] PENN STATE UNIV,DEPT CHEM,UNIVERSITY PK,PA 16802

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 02期

关键词：

Capillary electrophoresis; Giant dopamine cell; Intracellular voltammetry; Planorbis corneus;

D O I：

10.1111/j.1471-4159.1990.tb01918.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Voltammetric electrodes having a tip diameter of 2–12 μ and microscale capillary zone electrophoresis have been used for dynamic and static monitoring of dopamine in the somal cytoplasm of the giant dopamine neuron of Planorbis corneus. Intracellular dopamine levels can be altered by extracellular application of dopamine or ethanol. The latter experiment provides a means to estimate total stores of endogenous dopamine, and the evidence presented suggests that at least 98% of these stores are bound and not directly accessible to the cytoplasm. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：633 / 638

页数：6

共 22 条

[1]

BARBER A, 1986, COMP BIOCHEM PHYS C, V84, P171

[2] EXCITATORY, INHIBITORY AND BIPHASIC SYNAPTIC POTENTIALS MEDIATED BY AN IDENTIFIED DOPAMINE-CONTAINING NEURON [J].