MAPPING OF HUMAN CHROMOSOME-22 BY INSITU HYBRIDIZATION

被引：16

作者：

ZHANG, FR

AURIAS, A

DELATTRE, O

STERN, MH

BENITEZ, J

ROULEAU, G

THOMAS, G

机构：

[1] INST CURIE,BIOL SECT,CNRS,URA 620,26 RUE ULM,F-75231 PARIS 05,FRANCE

[2] USN HOSP,NCI USN MED ONCOL BRANCH,BETHESDA,MD 20814

[3] FDN JIMENEZ DIAZ,DEPT GENET,E-28040 MADRID,SPAIN

[4] MCGILL UNIV,MONTREAL GEN HOSP,MONTREAL H3G 1A4,QUEBEC,CANADA

来源：

GENOMICS | 1990年 / 7卷 / 03期

关键词：

D O I：

10.1016/0888-7543(90)90164-P

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The second smallest chromosome of the human karyotype, i.e., chromosome 22, is involved in many congenital or acquired structural aberrations. This variety can be taken advantage of to determine the exact linear order, from centromere to telomere, of cloned probes and chromosomal breakpoints. Eleven probes were localized with respect to breakpoints of 11 der(22) of independent cell lines using in situ hybridization on metaphasic spreads. The deduced order of the tested probes and that of the breakpoints are in complete agreement with the published genetic map and the karyotypic analysis, respectively. This approach enables a correlation of the genetic map with the chromosomal banding. © 1990.

引用

页码：319 / 324

页数：6

共 14 条

[1]

BUDARF M, 1989, AM J HUM GENET, V45, P128

[2] RECURRENT T(11-22) BREAKPOINT MAPPING BY CHROMOSOME FLOW SORTING AND SPOT-BLOT HYBRIDIZATION [J].