GENETIC AND SEQUENCE ANALYSES OF A PSEUDOMONAS-DENITRIFICANS DNA FRAGMENT CONTAINING 2 COB GENES

被引：23

作者：

CAMERON, B

GUILHOT, C

BLANCHE, F

CAUCHOIS, L

ROUYEZ, MC

RIGAULT, S

LEVYSCHIL, S

CROUZET, J

机构：

[1] RHONE POULENC RORER SA,CTR RECH VITRY ALFORTVILLE,INST BIOTECHNOL,UNITE BIOL MOLEC,F-94403 VITRY,FRANCE

[2] RHONE POULENC RORER SA,CTR RECH VITRY ALFORT,DEPT ANAL,F-94403 VITRY,FRANCE

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 19期

关键词：

D O I：

10.1128/jb.173.19.6058-6065.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A genetic analysis of a 12-kb DNA fragment containing Pseudomonas denitrificans cob genes was performed by transposon-mediated insertional mutagenesis. The nucleotide sequence and genetic analysis have shown that a 4.8-kb DNA subfragment carried two cob genes (cobS and cobT). Biochemical data concerning the complemented cobS and cobT mutants suggested that the cobS product was involved in cobalt insertion-mediating reactions and that the cobT product was involved in the transformation of precorrin-3 into cobyrinic acid.

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页码：6058 / 6065

页数：8

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