PROTON-NUCLEAR-MAGNETIC-RESONANCE-PH-TITRATION STUDIES OF THE HISTIDINES OF PANCREATIC PHOSPHOLIPASE-A2

被引：45

作者：

AGUIAR, A ^{[1
]}

DEHAAS, GH ^{[1
]}

JANSEN, EHJM ^{[1
]}

SLOTBOOM, AJ ^{[1
]}

WILLIAMS, RJP ^{[1
]}

机构：

[1] RIJKSUNIV UTRECHT,BIOCHEM LAB,NL-3584 CH UTRECHT,NETHERLANDS

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 100卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb04196.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The study by means of 1H nuclear magnetic resonance (NMR) of the histidines of phospholipase A2 isolated from porcine, bovine and equine pancreas is reported. Assignment of the histidine resonances was achieved by comparison of different enzymes and the use of paramagnetic probes. pH titration curves for various histidyl resonances were obtained and compared in the presence and absence of calcium. Calcium is shown to lower the pKa of the active site histidine. The NMR results are compared with the known X‐ray three‐dimensional structure for the bovine enzyme. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：511 / 518

页数：8

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