COMPARISON OF MOLECULAR EPIDEMIOLOGIC TOOLS FOR BRANHAMELLA-CATARRHALIS TYPING

被引：12

作者：

DENAMUR, E

PICARDPASQUIER, N

MURA, C

PICARD, B

ORFILA, J

KRISHNAMOORTHY, R

机构：

[1] FAC MED AMIENS,BACTERIOL IMMUNOL GEN LAB,F-80036 AMIENS,FRANCE

[2] INST PATHOL & BIOL CELLULAIRES & MOLEC,INSERM,U15,F-75674 PARIS 14,FRANCE

[3] UNIV PARIS 07,MICROBIOL LAB,F-75018 PARIS,FRANCE

来源：

RESEARCH IN MICROBIOLOGY | 1991年 / 142卷 / 05期

关键词：

BRANHAMELLA-CATARRHALIS; DNA; ESTERASE; ZYMOTYPE; RIBOTYPE; RFLP; EPIDEMIOLOGY;

D O I：

10.1016/0923-2508(91)90191-C

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Twenty-one Branhamella catarrhalis strains selected for their diversity by esterase electrophoretic polymorphism, and belonging to 20 distinct zymotypes, were studied by restriction fragment length polymorphism (RFLP) of total DNA after ethidium bromide staining and of ribosomal DNA regions (ribotyping). The former analysis allowed the distinction of 20 patterns and the latter the delineation of 19 ribotypes. The three methods were correlated and showed a clonal diversity of the species. Esterase electrophoresis and HinfI RFLP patterns after ethidium bromide staining appeared to be simple tools for use in B. catarrhalis epidemiology.

引用

页码：585 / 589

页数：5

共 13 条

[1] ESCHERICHIA-VULNERIS - A NEW SPECIES OF ENTEROBACTERIACEAE ASSOCIATED WITH HUMAN WOUNDS [J].