RAPID PCR CLONING AND SEQUENCE DETERMINATION OF THE RAT LITHOSTATHINE GENE

被引：6

作者：

DUSETTI, NJ ^{[1
]}

FRIGERIO, JM ^{[1
]}

DAGORN, JC ^{[1
]}

IOVANNA, JL ^{[1
]}

机构：

[1] INSERM,U315,46 BD GAYE,F-13009 MARSEILLE,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1174卷 / 01期

关键词：

PCR; GENE SEQUENCE; LITOSTATHINE GENE; (RAT);

D O I：

10.1016/0167-4781(93)90100-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rat lithostathine gene was isolated from a genomic library using a rapid screening procedure involving PCR amplification. It was characterized over 2.7 kbp of gene sequence and 2.43 kbp of 5'-flanking sequence. The 5'-end of the coding sequence was determined by primer extension of lithostathine mRNA. The lithostathine sequence spanned over six exons. The promoter region of the gene contained the TATAAA and CCAAT consensus sequences 30 and 107 bp upstream of the cap site, respectively. Furthermore, a tract of (TG)22 repeat, with potential Z-DNA conformation, was found at position - 1081.

引用

页码：99 / 102

页数：4

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