BIOCHEMICAL-IDENTIFICATION OF INTERBREEDING BETWEEN B-TYPE AND NON B-TYPE STRAINS OF THE TOBACCO WHITEFLY BEMISIA-TABACI

被引：39

作者：

BYRNE, FJ

CAHILL, M

DENHOLM, I

DEVONSHIRE, AL

机构：

[1] Department of Biological and Ecological Chemistry, Institute of Arable Crops Research, Harpenden, AL5 2JQ, Rothamsted Experimental Station

来源：

BIOCHEMICAL GENETICS | 1995年 / 33卷 / 1-2期

关键词：

WHITEFLY BIOTYPES; INTERBREEDING; HETEROZYGOTES; B-TYPE; BEMISIA TABACI;

D O I：

10.1007/BF00554554

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyacrylamide gel electrophoresis and a kinetic microplate assay were used to detect heterozygotes resulting from a cross between B-type and non B-type strains of the whitefly Bemisia tabaci. Both strains were homozygous for different esterase and acetylcholinesterase (AChE) enzymes, and heterozygotes were produced in one of two crosses between B-type females (diploid) and non B-type males (haploid). In reciprocal crosses, however, no female offspring were produced indicating that fertilization had not occurred Despite the identification of individuals heterozygous for the esterase and AChE markers, there was clearly a significant degree of reproductive incompatibility between the two strains. The biochemical assays provided a vital component of this study and the advantages of their use are discussed.

引用

页码：13 / 23

页数：11

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