ON SPECIFICITY OF ALCHOLIC, ACIDIC SILVER NITRATE REAGENT FOR HISTOCHEMICAL LOCALIZATION OF ASCORBIC ACID

被引：39

作者：

CHINOY, NJ

机构：

[1] Department of Zoology, School of Sciences, Gujarat University, Ahmedabad

来源：

HISTOCHEMIE | 1969年 / 20卷 / 02期

关键词：

D O I：

10.1007/BF00268703

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The defects besetting the histochemical localization of ascorbic acid were removed in the modified method described here by the simultaneous fixation of the experimental material and its reaction with silver nitrate by the use of alcoholic, acidic silver nitrate reagent in the dark at 0-3°C for 24 hours or longer at pH 2-2.5. The fixatives like acetic acid and alcohol of the reagent ensure quick penetration of AgNO3 for fixation of ascorbic acid in situ before sectioning. It has been experimentally established that none of the other reductants react with AgNO3 at the pH and the temperature mentioned. The sections were devitaminized by treatment with 6-10% formaline for 3-4 hours to serve as a control. © 1969 Springer-Verlag.

引用

页码：105 / &

共 16 条

[1] CYTOCHEMICAL STUDY OF THE ADRENAL CORTEX OF THE RAT UNDER SALT STRESSES [J].