REGULATION OF TRANSCRIPTION OF THE PROSTATE GENOME BY ANDROGENS

被引:21
作者
DAVIES, P
THOMAS, P
GILES, MG
BOONJAWAT, J
GRIFFITHS, K
机构
[1] Tenovus Institute for Cancer Research, Welsh National School of Medicine, Heath Park, Cardiff
关键词
D O I
10.1016/0022-4731(79)90053-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effect of androgen withdrawal on a number of parameters of transcription has been studied using reconstituted systems based on rat ventral prostate chromatin or nuclei. Nuclear receptors, total RNA polymerase activity, the numbers of RNA polymerase molecules active in transcription, RNA-chain lengths and the rates of their elongation are all significantly decreased 1 day after castration and continue to decrease, less dramatically, until 7 days after castration. Both α-amanitin-sensitive and -insensitive RNA polymerase activities were affected. The changes in initiation sites on chromatin available to RNA polymerase B do not correlate with alterations in receptor levels and RNA synthesis in intact nuclei. Possible reasons for these discrepancies are discussed. Hybridization of cellular poly(A)-containing RNA from prostates of normal rats and rats castrated 3 days previously suggested that castration resulted in the reduced concentration of a small number of highly-abundant poly(A) RNA sequences. © 1979.
引用
收藏
页码:351 / 360
页数:10
相关论文
共 51 条
[2]   GLOBIN RNA PRECURSOR MOLECULES - BIOSYNTHESIS AND PROCESSING IN ERYTHROID-CELLS [J].
BASTOS, RN ;
AVIV, H .
CELL, 1977, 11 (03) :641-650
[3]   3 ABUNDANCE CLASSES IN HELA-CELL MESSENGER-RNA [J].
BISHOP, JO ;
MORTON, JG ;
ROSBASH, M ;
RICHARDSON, M .
NATURE, 1974, 250 (5463) :199-204
[4]  
Bruchovsky N, 1975, Vitam Horm, V33, P61
[5]   STUDIES ON REGULATION OF CONCENTRATION OF ANDROGENS AND ANDROGEN RECEPTORS IN NUCLEI OF PROSTATIC CELLS [J].
BRUCHOVSKY, N ;
RENNIE, PS ;
VANSON, A .
BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 394 (02) :248-266
[6]   UNSTABLE NUCLEAR RNA SYNTHESIS FOLLOWING ESTROGEN STIMULATION [J].
CHURCH, RB ;
MCCARTHY, BJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1970, 199 (01) :103-&
[7]   MECHANISM BY WHICH HEPARIN STIMULATES TRANSCRIPTION IN ISOLATED RAT-LIVER NUCLEI - POLYRIBONUCLEOTIDE ELONGATION RATES AND NUMBER OF TRANSCRIBING RNA-POLYMERASE MOLECULES PRESENT [J].
COUPAR, BEH ;
CHESTERTON, CJ .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 79 (02) :525-533
[8]   QUANTITATION OF ELONGATING FORM-A AND FORM-B RNA POLYMERASES IN CHICK OVIDUCT NUCLEI AND EFFECTS OF ESTRADIOL [J].
COX, RF .
CELL, 1976, 7 (03) :455-465
[9]   MEASUREMENT OF FREE AND OCCUPIED CYTOPLASMIC AND NUCLEAR ANDROGEN RECEPTOR-SITES IN RAT VENTRAL PROSTATE-GLAND [J].
DAVIES, P ;
THOMAS, P ;
GRIFFITHS, K .
JOURNAL OF ENDOCRINOLOGY, 1977, 74 (03) :393-404
[10]   STIMULATION OF RIBONUCLEIC-ACID POLYMERASE-ACTIVITY IN-VITRO BY PROSTATIC STEROID-PROTEIN RECEPTOR COMPLEXES [J].
DAVIES, P ;
GRIFFITH.K .
BIOCHEMICAL JOURNAL, 1973, 136 (03) :611-622