OPTIMIZATION OF THE ANALYTICAL OXIDATION OF DIHYDRONICOTINAMIDE ADENINE-DINUCLEOTIDE AT CARBON AND PLATINUM-ELECTRODES

Much of the difficulty associated with the voltammetric determination of NADH at carbon electrodes (glassy carbon and pyrolytic graphite) can be eliminated by first covering the electrode surface with adsorbed electrochemically generated NAD+. Such an electrode can be repeatedly used for NADH measurement without the necessity for pretreatment. At the millimolar NADH level, analytically satisfactory results can be obtained by simply comparing the test solution current at one potential on the limiting current plateau for the RDE with the background solution current at the same potential; a comparably simple procedure can also be used at the micromolar level. Results obtained at rotating and stationary glassy carbon, pyrolytic graphite, platinum electrodes are compared. © 1979, American Chemical Society. All rights reserved.