EFFECT OF RABBIT STRAIN ON ACTIVITY LEVEL AND CYTOTOXICITY OF SERUM COMPLEMENT .3. COMPARISON OF 4 TUMOR TARGET-CELLS

Serum samples from about 10 males and 10 females from each of 15 genetically defined strains of rabbits and from one hybrid were tested as sources of complement for the microtiter lymphocytotoxicity test using tumor cells from Sal-A, 6C3HED-A, BW5147, and L-cells as target cells. The tumor cells were tested with appropriate H-2 antisera. Results indicated strain differences particularly evident when the target cells were either 6C3HED-A or L-cells. BW5147 worked well with most all strains and Sal-A was extremely demanding of all strains of rabbits. However, strains IIIC/J, IIIVO/J and the F1 hybrid between them were still among the six best strains when tested against the tumor cells as they were when tested against the lymph nodes in previously published data.Strain WH/J which carries the gene, ha, for hereditary lymphosarcoma, proved to be a good complement source with Sal-A and 6C3HED-A, both sarcoma in nature. However, it was not a good complement source with BW5 147, a lymphatic leukemia, or L-cells. This is possible evidence for a common tumor antigen associated with lymphosarcoma. © 1979 AMERICAN GENETIC ASSOCIATION.