INTERACTION OF ITURIN-A, A LIPOPEPTIDE ANTIBIOTIC, WITH SACCHAROMYCES-CEREVISIAE CELLS - INFLUENCE OF THE STEROL MEMBRANE-COMPOSITION

被引：22

作者：

LATOUD, C ^{[1
]}

PEYPOUX, F ^{[1
]}

MICHEL, G ^{[1
]}

机构：

[1] UNIV LYON 1,BIOCHIM MICROBIENNE LAB,CNRS,UNITE RECH ASSOCIEE 1176,F-69622 VILLEURBANNE,FRANCE

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1990年 / 36卷 / 06期

关键词：

antifungal lipopeptide; iturin A; membrane sterol; yeast cell binding capacity;

D O I：

10.1139/m90-067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The binding of the membrane-active lipopeptide antibiotic iturin A to yeast cells was studied using radioactive iturin A. Saccharomyces cerevisiae had a maximum binding capacity of 5.6 x 109 molecules per single cell. The Scatchard plot of binding showed a biphasic profile, with a lower dissociation constant for small concentrations of iturin A. The break of slope at 30 μM iturin A corresponds to the micellization of the antibiotic in solution. The binding is also dependent on the nature of the sterol present in the membrane. A mutant yeast strain with a membrane containing cholesterol instead of ergosterol showed the highest affinity for iturin A and the highest sensitivity to this antibiotic, as measured by K+ ion release. In contrast the presence of stigmasterol increased the resistance of the cells to iturin A.

引用

页码：384 / 389

页数：6

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