USE OF INVIVO AND INVITRO ASSAYS FOR THE CHARACTERIZATION OF MAMMALIAN EXCISION REPAIR AND ISOLATION OF REPAIR PROTEINS

被引：37

作者：

HOEIJMAKERS, JHJ ^{[1
]}

EKER, APM ^{[1
]}

WOOD, RD ^{[1
]}

ROBINS, P ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND, CLARE HALL LABS, S MIMMS EN6 3LD, HERTS, ENGLAND

来源：

MUTATION RESEARCH | 1990年 / 236卷 / 2-3期

关键词：

DNA repair; Nucleotide excision repair; Proteins; in DNA repair; Xeroderma pigmentosum;

D O I：

10.1016/0921-8777(90)90007-R

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Elucidation of the molecular mechanism of mammalian nucleotide excision repair requires the availability of purified proteins, DNA substrates with defined lesions and suitable repair assays. Repair assays introduced in recent years vary from testing individual steps and successions of steps in vitro to systems that closely reflect the entire process in vivo. In the first part of this review, an in vivo microinjection system is discussed. The second part of the article reviews an in vitro system for study of repair synthesis promoted by cell extracts. Both systems can be utilized as assays during the purification of protein factors that complement repair-defective xeroderma pigmentosum cells. The effect of purified repair proteins from other organisms on mammalian repair is also considered. © 1990.

引用

页码：223 / 238

页数：16

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