A TRANSFERRIN-BINDING PROTEIN OF TRYPANOSOMA-BRUCEI IS ENCODED BY ONE OF THE GENES IN THE VARIANT SURFACE GLYCOPROTEIN GENE-EXPRESSION SITE

被引：107

作者：

SCHELL, D ^{[1
]}

EVERS, R ^{[1
]}

PREIS, D ^{[1
]}

ZIEGELBAUER, K ^{[1
]}

KIEFER, H ^{[1
]}

LOTTSPEICH, F ^{[1
]}

CORNELISSEN, AWCA ^{[1
]}

OVERATH, P ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM, W-8033 MARTINSRIED, GERMANY

来源：

EMBO JOURNAL | 1991年 / 10卷 / 05期

关键词：

EXPRESSION SITE ASSOCIATED GENE; GLYCOSYLPHOSPHATIDYLINOSITOL; TRANSFERRIN-BINDING PROTEIN; TRYPANOSOMA-BRUCEI; VARIANT SURFACE GLYCOPROTEIN;

D O I：

10.1002/j.1460-2075.1991.tb08045.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A transferrin-binding protein (TFBP) with an apparent molecular weight of 42 kd was purified from detergent-soluble membrane proteins of bloodstream forms of Trypanosoma brucei. The protein is not expressed in the insect-borne stage of the parasite's life-cycle. Purified TFBP can be converted from an amphiphillic to a hydrophilic form by cleavage with T. brucei glycosylphophatidylinositol (GPI)-specific phospholipase C, demonstrating that the C-terminus is modified by a GPI-membrane anchor. The TFBP is encoded by an expression-site-associated gene [ESAG 6 in the nomenclature of Pays et al. (1989) Cell, 57, 835-845] which is under the control of the promoter transcribing the expressed variant surface glycoprotein gene. The possible function of TFBP as a receptor for the uptake of transferrin in bloodstream forms is discussed.

引用

页码：1061 / 1066

页数：6

共 58 条

[1] PUTATIVE GENES OF A VARIANT-SPECIFIC ANTIGEN GENE-TRANSCRIPTION UNIT IN TRYPANOSOMA-BRUCEI [J].