CA2+ WAVE-PROPAGATION IN CELLS OF PANCREATIC ACINUS REVERSES AFTER CELL DISPERSION

Digital image analysis with fura-2 was made to investigate early stage of secretagogue-induced [Ca2+]c increase in pancreatic acinar cells. When isolated acini dissociated with collagenase were stimulated with 10 pM CCK-8 or 10 nM ACh, the initial rise in [Ca2+]c occurred in the region beneath the basolateral plasma membrane. When the acinar cells isolated with collagenase, trypsin and EGTA were stimulated with 1 mM ACh, the rise started at the luminal region. Morphological deteriorations were detected in the latter preparation. Possible causes for the opposite [Ca2+]c gradient may be in procedures of isolation, destruction of acinar constitution, and composition of perifusing solution.