EFFECT OF ETHYLENE GLYCOL-BIS(BETA-AMINOETHYL ETHER)-N,N-TETRAACETIC ACID (EGTA) ON STABILITY AND ACTIVITY OF METHANOGENIC GRANULAR SLUDGE

被引:43
作者
GROTENHUIS, JTC [1 ]
VANLIER, JB [1 ]
PLUGGE, CM [1 ]
STAMS, AJM [1 ]
ZEHNDER, AJB [1 ]
机构
[1] WAGENINGEN UNIV AGR,CTR BIOMOLEC SCI,DEPT MICROBIOL,HESSELINK VAN SUCHTELENWEG 4,6703 CT WAGENINGEN,NETHERLANDS
关键词
D O I
10.1007/BF00164709
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effect of the calcium-specific chelant ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid (EGTA) on methanogenic granular sludge from a laboratory-scale upflow anaerobic sludge-blanket (UASB) reactor fed propionate and from a full-scale reactor treating paper-mill waste-water was studied. Upon treatment with EGTA both sludge types showed a decrease in the calcium and phosphorus content and a release of protein and polysaccharides, leading to a decrease in strength of papermill granular sludge and a disintegration of propionate-grown granules. After treatment of propionate-grown granular sludge with high EGTA concentrations, the methanogenic activity with propionate and acetate as test substrates decreased by 88 and 33%, respectively. The marked reduction in propionate oxidation activity may be caused by a disruption of the special juxtapositioning of bacteria in the granules.
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页码:109 / 114
页数:6
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