REGENERATION OF HERBICIDE RESISTANT TRANSGENIC RICE PLANTS FOLLOWING MICROPROJECTILE-MEDIATED TRANSFORMATION OF SUSPENSION-CULTURE CELLS

被引：63

作者：

CAO, J

DUAN, XL

MCELROY, D

WU, R

机构：

[1] CORNELL UNIV, FIELD BOT, ITHACA, NY 14853 USA

[2] CORNELL UNIV, CTR PLANT SCI, ITHACA, NY 14853 USA

[3] CORNELL UNIV, DEPT BIOCHEM MOLEC & CELL BIOL, ITHACA, NY 14853 USA

来源：

PLANT CELL REPORTS | 1992年 / 11卷 / 11期

关键词：

TRANSGENIC RICE; HERBICIDE RESISTANCE; PHOSPHINOTHRICIN ACETYL TRANSFERASE GENE;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Suspension cells of Oryza sativa L. (rice) were transformed, by microprojectile bombardment, with plasmids carrying the coding region of the Streptomyces hygroscopicus phosphinothricin acetyl transferase (PAT) gene (bar) under the control of either the 5' region of the rice actin 1 gene (Act1) or the cauliflower mosaic virus (CaMV) 35S promoter. Subsequently regenerated plants display detectable PAT activity and are resistant to BASTA(TM), a phosphinothricin (PPT)-based herbicide. DNA gel blot analyses showed that PPT resistant rice plants contain a bar-hybridizing restriction fragment of the expected size. This report shows that expression of the bar gene in transgenic rice plants confers resistance to PPT-based herbicide by suppressing an increase of ammonia in plants after spraying with the herbicide.

引用

页码：586 / 591

页数：6

共 19 条

[1]

Cao J., 1991, Rice biotechnology., P175

[2]

CAO J, 1990, PLANT GENE TRANSFER, P21

[3] PRODUCTION OF TRANSGENIC RICE (ORYZA-SATIVA L) PLANTS FROM AGRONOMICALLY IMPORTANT INDICA AND JAPONICA VARIETIES VIA ELECTRIC-DISCHARGE PARTICLE-ACCELERATION OF EXOGENOUS DNA INTO IMMATURE ZYGOTIC EMBRYOS [J].