REQUIREMENT OF PHOSPHATIDYLGLYCEROL FOR FLAGELLATION OF ESCHERICHIA-COLI

被引：16

作者：

TOMURA, A ^{[1
]}

ISHIKAWA, T ^{[1
]}

SAGARA, Y ^{[1
]}

MIKI, T ^{[1
]}

SEKIMIZU, K ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC PHARMACEUT SCI,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

FEBS LETTERS | 1993年 / 329卷 / 03期

关键词：

PHOSPHATIDYLGLYCEROL; BACTERIAL MOTILITY; FLAGELLUM; FLAGELLIN; ESCHERICHIA-COLI;

D O I：

10.1016/0014-5793(93)80239-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report that phosphatidylglycerol is required for flagellation of Escherichia coli. Cells carrying the pgsA3 mutation did not form swarm rings in semisolid agar. P1 transduction experiments revealed that the potential for phosphatidylglycerol synthesis and for the formation of swarm rings was co-transducible. The pgsA3 mutant transformed with the wild type pgsA+ gene cloned into the R-plasmid vector had the potential for both phosphatidylglycerol synthesis and cell motility. Electronmicroscopic and SDS-PAGE analyses showed that the pgsA3 mutation causes the lack of flagellation.

引用

页码：287 / 290

页数：4

共 15 条

[1] LIPIDS OF SALMONELLA TYPHIMURIUM AND ESCHERICHIA COLI - STRUCTURE AND METABOLISM [J].

AMES, GF .

JOURNAL OF BACTERIOLOGY, 1968, 95 (03) :833-+

[2] NONCHEMOTACTIC MUTANTS OF ESCHERICHIA COLI [J].

ARMSTRONG, JB ;

ADLER, J ;

DAHL, MM .

JOURNAL OF BACTERIOLOGY, 1967, 93 (01) :390-+

[3] SUPPRESSION OF THE LETHAL EFFECT OF ACIDIC-PHOSPHOLIPID DEFICIENCY BY DEFECTIVE FORMATION OF THE MAJOR OUTER-MEMBRANE LIPOPROTEIN IN ESCHERICHIA-COLI [J].

ASAI, Y ;

KATAYOSE, Y ;

HIKITA, C ;

OHTA, A ;

SHIBUYA, I .

JOURNAL OF BACTERIOLOGY, 1989, 171 (12) :6867-6869

[4]

EINSTEIN BI, 1987, ESCHERICHIA COLI SAL, P84

[5]

GOPALAKRISHNAN AS, 1986, J BIOL CHEM, V261, P1329

[6] ISOLATION AND CHARACTERIZATION OF ESCHERICHIA-COLI HAG OPERATOR MUTANTS WHOSE HAG48 EXPRESSION HAS BECOME REPRESSIBLE BY A SALMONELLA H1-REPRESSOR [J].

HANAFUSA, T ;

SAKAI, A ;

TOMINAGA, A ;

ENOMOTO, M .

MOLECULAR AND GENERAL GENETICS, 1989, 216 (01) :44-50

[7] PROCESS OF CELLULAR DIVISION IN ESCHERICHIA-COLI - MUTANT OF ESCHERICHIA-COLI LACKING A MUREIN-LIPOPROTEIN .10. [J].

HIROTA, Y ;

SUZUKI, H ;

NISHIMURA, Y ;

YASUDA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (04) :1417-1420

[8] NUCLEOTIDE-SEQUENCE OF THE HAG GENE ENCODING FLAGELLIN OF ESCHERICHIA-COLI [J].

KUWAJIMA, G ;

ASAKA, JI ;

FUJIWARA, T ;

NODE, K ;

KONDO, E .

JOURNAL OF BACTERIOLOGY, 1986, 168 (03) :1479-1483

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] CONTROL OF SEGREGATION OF CHROMOSOMAL DNA BY SEX FACTOR-F IN ESCHERICHIA-COLI - MUTANTS OF DNA GYRASE SUBUNIT-A SUPPRESS LETD (CCDB) PRODUCT GROWTH-INHIBITION [J].

MIKI, T ;

PARK, JA ;

NAGAO, K ;

MURAYAMA, N ;

HORIUCHI, T .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 225 (01) :39-52

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