DIRECT MEASUREMENTS OF NATURAL PLANKTONIC BACTERIAL COMMUNITY VIABILITY BY FLOW-CYTOMETRY

被引：74

作者：

PORTER, J ^{[1
]}

DIAPER, J ^{[1
]}

EDWARDS, C ^{[1
]}

PICKUP, R ^{[1
]}

机构：

[1] INST FRESHWATER ECOL, WINDERMERE LAB, AMBLESIDE LA22 0LP, CUMBRIA, ENGLAND

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 07期

关键词：

D O I：

10.1128/AEM.61.7.2783-2786.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A range of fluorescent viability dyes were used in conjunction with flow cytometry to rapidly enumerate viable bacteria from freshwater environments. Optimal labelling was achieved by using carboxyfluorescein diacetate or chemchrome B with a detergent-mediated permeabilization step. The viable bacterial count under optimal conditions was 7% in oligotrophic lake water and 75% in polluted river water.

引用

页码：2783 / 2786

页数：4

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