IMMUNOFIXATION ON CELLULOSE-ACETATE - IMPROVED SCREENING METHOD FOR MONOCLONAL IMMUNOGLOBULINS

被引：14

作者：

PIZZOLATO, MA

GONI, FR

SALVAREZZA, RC

机构：

[1] Department of Clinical Chemistry, Faculty of Biochemistry, University of Buenos Aires, 1113 Buenos Aires

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 26卷 / 04期

关键词：

D O I：

10.1016/0022-1759(79)90182-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, simple and economical method is described for typing monoclonal immunoglobulins. It is a modification of the immunofixation electrophoresis method and cellulose acetate has been used as a supporting medium. It involves an initial electrophoretic separation followed by an antigen (Ag)-antibody (Ab) reaction 'in situ'. Eight samples can be typed on each 5.7 cm × 10.5 cm strip and only 20 μl of commercial antiserum are required (about 2.5 μl per sample). The method permits detection of monoclonal proteins at concentrations as low as 100 ng/μl in only 60 min. © 1979.

引用

页码：365 / 368

页数：4

共 11 条

[1] IMMUNOFIXATION ELECTROPHORESIS - A TECHNIQUE FOR STUDY OF PROTEIN POLYMORPHISM [J].