INTERLEUKIN-6, A POSSIBLE AUTOCRINE GROWTH AND DIFFERENTIATION FACTOR FOR THE HUMAN MEGAKARYOCYTIC CELL-LINE, CMK

被引：33

作者：

FUSE, A ^{[1
]}

KAKUDA, H ^{[1
]}

SHIMA, Y ^{[1
]}

VANDAMME, J ^{[1
]}

BILLIAU, A ^{[1
]}

SATO, T ^{[1
]}

机构：

[1] CHIBA UNIV,SCH MED,DEPT MICROBIOL & PEDIAT,CHIBA,JAPAN

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1991年 / 77卷 / 01期

关键词：

D O I：

10.1111/j.1365-2141.1991.tb07944.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

CMK is a human cell line derived from a megakaryoblastic leukaemia. It has characteristics of the megakaryocytic lineage, such as the presence of platelet peroxidase, membrane glycoproteins (GP)Ib and GPIIb/IIIa, alpha-granules, and demarcation membranes. The cell line proliferates autonomously in serum-containing medium. Here we report that the cell line expresses the gene for IL-6 and releases small quantities of the cytokine into the medium. Addition of exogenous IL-6 to cultures seeded into medium was found to promote growth of the cells. Conversely, addition of a neutralizing anti-IL-6 antibody inhibited cell growth. These data support the notion that autocrine IL-6 is one of the factors accounting for autonomous growth of the cell line.

引用

页码：32 / 36

页数：5

共 25 条

[1] ADACHI N, 1988, ACTA HAEMATOLOGICA J, V51, P1069
[2] BRUNO E, 1989, EXP HEMATOL, V17, P1038
[3] INTERLEUKIN-1 AS AN AUTOCRINE GROWTH-FACTOR FOR ACUTE MYELOID-LEUKEMIA CELLS
COZZOLINO, F
RUBARTELLI, A
ALDINUCCI, D
SITIA, R
TORCIA, M
SHAW, A
DIGUGLIELMO, R
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (07) : 2369 - 2373
[4] INTERFERON BETA-2/B-CELL STIMULATORY FACTOR TYPE-2 SHARES IDENTITY WITH MONOCYTE-DERIVED HEPATOCYTE-STIMULATING FACTOR AND REGULATES THE MAJOR ACUTE PHASE PROTEIN RESPONSE IN LIVER-CELLS
GAULDIE, J
RICHARDS, C
HARNISH, D
LANSDORP, P
BAUMANN, H
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) : 7251 - 7255
[5] STRUCTURAL-ANALYSIS OF THE SEQUENCE CODING FOR AN INDUCIBLE 26-KDA PROTEIN IN HUMAN-FIBROBLASTS
HAEGEMAN, G
CONTENT, J
VOLCKAERT, G
DERYNCK, R
TAVERNIER, J
FIERS, W
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 159 (03): : 625 - 632
[6] COMPLEMENTARY-DNA FOR A NOVEL HUMAN INTERLEUKIN (BSF-2) THAT INDUCES LYMPHOCYTES-B TO PRODUCE IMMUNOGLOBULIN
HIRANO, T
YASUKAWA, K
HARADA, H
TAGA, T
WATANABE, Y
MATSUDA, T
KASHIWAMURA, S
NAKAJIMA, K
KOYAMA, K
IWAMATSU, A
TSUNASAWA, S
SAKIYAMA, F
MATSUI, H
TAKAHARA, Y
TANIGUCHI, T
KISHIMOTO, T
[J]. NATURE, 1986, 324 (6092) : 73 - 76
[7] HOMANS AC, 1989, NOUVELLE REV FRANCAI, V31, P239
[8] INTERLEUKIN-6 ENHANCEMENT OF INTERLEUKIN-3-DEPENDENT PROLIFERATION OF MULTIPOTENTIAL HEMATOPOIETIC PROGENITORS
IKEBUCHI, K
WONG, GG
CLARK, SC
IHLE, JN
HIRAI, Y
OGAWA, M
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (24) : 9035 - 9039
[9] HUMAN INTERLEUKIN-6 IS A DIRECT PROMOTER OF MATURATION OF MEGAKARYOCYTES INVITRO
ISHIBASHI, T
KIMURA, H
UCHIDA, T
KARIYONE, S
FRIESE, P
BURSTEIN, SA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (15) : 5953 - 5957
[10] AUTOCRINE GENERATION AND REQUIREMENT OF BSF-2/IL-6 FOR HUMAN MULTIPLE MYELOMAS
KAWANO, M
HIRANO, T
MATSUDA, T
TAGA, T
HORII, Y
IWATO, K
ASAOKU, H
TANG, B
TANABE, O
TANAKA, H
KURAMOTO, A
KISHIMOTO, T
[J]. NATURE, 1988, 332 (6159) : 83 - 85

← 1 2 3 →