SYNTHESIS OF THIOMORPHOLINO BUFFERS FOR ISOELECTRIC-FOCUSING IN IMMOBILIZED PH GRADIENTS

被引：11

作者：

CHIARI, M

RIGHETTI, PG

FERRABOSCHI, P

JAIN, T

SHORR, R

机构：

[1] UNIV MILAN, DEPT BIOMED SCI & TECHNOL, VIA CELORIA 2, I-20133 MILAN, ITALY

[2] AT BIOCHEM, MALVERN, PA USA

[3] UNIV MILAN, FAC PHARM, INST ENDOCRINOL, I-20122 MILAN, ITALY

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 08期

关键词：

D O I：

10.1002/elps.1150110806

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The two commercially available Immobilines having a pK of 6.2 (2‐morpholino ethyl acrylamide) and 7.0 (3‐morpholinopropylacrylamide) have been modified and two new buffers have been synthesized: 2‐thiomorpholinoethylacrylamide, pK 6.6, and 3‐thiomorpholinopropyl acrylamide, pK 7.4. The replacement of an oxygen with a sulfur atom in the morpholino ring is thus seen to shift the pK values of these two bases by +0.4 pH units. In formulations in which the two new bases replaced the standard morpholino derivatives, identical pH profiles and protein patterns were obtained. The reason for this work was to try to close the gap between the pK 7.0 and 8.5 species and to provide the users of immobilized pH gradients with more buffers in the neutral pH region. The two new thiomorpholino derivatives are an important step in this direction. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：617 / 620

页数：4

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