POSSIBLE SECONDARY STRUCTURE IN PLANT AND YEAST BETA-GLUCANASE

被引：10

作者：

MACGREGOR, EA

BALLANCE, GM

机构：

[1] UNIV MANITOBA,DEPT PLANT SCI,WINNIPEG R3T 2N2,MANITOBA,CANADA

[2] UNIV MANITOBA,DEPT CHEM,WINNIPEG R3T 2N2,MANITOBA,CANADA

来源：

BIOCHEMICAL JOURNAL | 1991年 / 274卷

关键词：

D O I：

10.1042/bj2740041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Circular-dichroism spectra of a barley 1,3-beta-glucanase were analysed by two methods. The combined results predict 36-40% helix and 15-18% beta-structure in the protein. Prediction of secondary-structural features on the basis of amino acid sequence information yielded overall helix and beta-structure contents of 37% and 19% respectively. Comparison of the predicted structural elements along the barley 1,3-beta-glucanase with those of three related plant glucanases and a yeast glucanase suggest a close similarity in secondary structure among the five proteins. Consideration is given to the potential importance of certain amino acids which are conserved in these five glucanases.

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页码：41 / 43

页数：3

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