INHIBITION OF THE NEUTROPHIL OXIDATIVE RESPONSE TO A CHEMOTACTIC PEPTIDE BY INHIBITORS OF ARACHIDONIC-ACID OXYGENATION

被引：83

作者：

BOKOCH, GM

REED, PW

机构：

[1] Department of Pharmacology, Vanderbilt University School of Medicine, Nashville

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 02期

关键词：

D O I：

10.1016/0006-291X(79)91260-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-formylmethionylphenylalanine stimulates a short burst of antimycin A-insensitive O2 uptake, O2- production and hexosemonophosphate shunt oxidation of glucose by guinea pig peritoneal neutrophils. The stimulated oxidative metabolism, as well as release of lysosomal enzymes ± cytochalasin B, are inhibited by 5,8,11,14-eicosatetraynoic acid (ID50 1.5 × 10-5 M). High concentrations of indomethacin inhibit the peptide-stimulated oxidations (ID50 1.6 × 10-4 M) while acetylsalicylic acid (2.5 × 10-3 M) does not. Digitonin-stimulated oxidative metabolism and enzyme release are not inhibited by 5,8,11,14-eicosatetraynoic acid or indomethacin at concentrations that depress effects of the N-formylated peptide. © 1979.

引用

页码：481 / 487

页数：7

共 13 条

[1]

BORGEAT P, 1979, J BIOL CHEM, V254, P2643

[2] SUPEROXIDE PRODUCTION BY DIGITONIN-STIMULATED GUINEA-PIG GRANULOCYTES - EFFECTS OF N-ETHYL MALEIMIDE, DIVALENT-CATIONS, AND GLYCOLYTIC AND MITOCHONDRIAL INHIBITORS ON ACTIVATION OF SUPEROXIDE GENERATING SYSTEM [J].