VOLTAGE-DEPENDENT CALCIUM CHANNELS IN PLANT VACUOLES

被引：69

作者：

PANTOJA, O ^{[1
]}

GELLI, A ^{[1
]}

BLUMWALD, E ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT BOT,CTR PLANT BIOTECHNOL,TORONTO M5S 3B2,ONTARIO,CANADA

来源：

SCIENCE | 1992年 / 255卷 / 5051期

关键词：

D O I：

10.1126/science.255.5051.1567

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Free calcium (Ca2+) in the cytoplasm of plant cells is important for the regulation of many cellular processes and the transduction of stimuli. Control of cytoplasmic Ca2+ involves the activity of pumps, carriers, and possibly ion channels. The patch-clamp technique was used to study Ca2+ channels in the vacuole of sugar beet cells. Vacuolar currents showed inward rectification at negative potentials, with a single-channel conductance of 40 picosiemens and an open probability dependent on potential. Channels were inhibited by verapamil and lanthanum. These channels could participate in the regulation of cytoplasmic Ca2+ by sequestering Ca2+ inside the vacuole.

引用

页码：1567 / 1570

页数：4

共 22 条

[1] OPENING OF CA-2+ CHANNELS IN ISOLATED RED BEET ROOT VACUOLE MEMBRANE BY INOSITOL 1,4,5-TRISPHOSPHATE [J].

ALEXANDRE, J ;

LASSALLES, JP ;

KADO, RT .

NATURE, 1990, 343 (6258) :567-570

[2] INOSITOL PHOSPHATES AND CELL SIGNALING [J].

BERRIDGE, MJ ;

IRVINE, RF .

NATURE, 1989, 341 (6239) :197-205

[3]

BRAUER M, 1990, PLANTA, V182, P236, DOI 10.1007/BF00197117

[4] THE CALMODULIN-STIMULATED ATPASE OF MAIZE COLEOPTILES IS A 140000-MR POLYPEPTIDE [J].

BRIARS, SA ;

KESSLER, F ;

EVANS, DE .

PLANTA, 1988, 176 (02) :283-285

[5] INTRACELLULAR CALCIUM HOMEOSTASIS [J].

CARAFOLI, E .

ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :395-433

[6] PHOTOTROPISM AND GEOTROPISM IN MAIZE COLEOPTILES ARE SPATIALLY CORRELATED WITH INCREASES IN CYTOSOLIC FREE CALCIUM [J].

GEHRING, CA ;

WILLIAMS, DA ;

CODY, SH ;

PARISH, RW .

NATURE, 1990, 345 (6275) :528-530

[7] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].

HAMILL, OP ;

MARTY, A ;

NEHER, E ;

SAKMANN, B ;

SIGWORTH, FJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100

[8] CYTOPLASMIC CALCIUM REGULATES VOLTAGE-DEPENDENT ION CHANNELS IN PLANT VACUOLES [J].

HEDRICH, R ;

NEHER, E .

NATURE, 1987, 329 (6142) :833-836

[9] DIFFERENT MODES OF CA-CHANNEL GATING BEHAVIOR FAVORED BY DIHYDROPYRIDINE CA-AGONISTS AND ANTAGONISTS [J].

HESS, P ;

LANSMAN, JB ;

TSIEN, RW .

NATURE, 1984, 311 (5986) :538-544

[10] CALCIUM-CHANNEL SELECTIVITY FOR DIVALENT AND MONO-VALENT CATIONS - VOLTAGE AND CONCENTRATION-DEPENDENCE OF SINGLE CHANNEL CURRENT IN VENTRICULAR HEART-CELLS [J].

HESS, P ;

LANSMAN, JB ;

TSIEN, RW .

JOURNAL OF GENERAL PHYSIOLOGY, 1986, 88 (03) :293-319

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