Y-CHROMOSOME LOSS IN ESOPHAGEAL-CARCINOMA - AN IN-SITU HYBRIDIZATION STUDY

被引:53
作者
HUNTER, S [1 ]
GRAMLICH, T [1 ]
ABBOTT, K [1 ]
VARMA, V [1 ]
机构
[1] EMORY UNIV,SCH MED,ATLANTA,GA 30322
关键词
D O I
10.1002/gcc.2870080306
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Carcinoma of the esophagus shows a strong male predominance and other epidemiologic differences from cancers arising at other sites. In this study, the prevalence of Y chromosome loss in 29 carcinomas of the esophagus and 53 carcinomas arising elsewhere in the aerodigestive tract was assessed by in situ hybridization of formalin-fixed paraffin-embedded tissue sections. Absence of the Y chromosome was defined as (1) negative staining for Y in neoplastic cells with positive staining for Y in immediately adjacent nonneoplastic epithelial and stromal cells, (2) positive staining of neoplastic cells with control probes for chromosomes X and 17, and (3) similar results at different stringencies and levels of protein digestion. According to these criteria, absence of the Y chromosome was observed in 13 of 14 (93%) adenocarcinomas of the esophagus, 8 of 13 (62%) squamous cell carcinomas of the esophagus, and 5 of 53 (9%) carcinomas arising in other sites. For the neoplasms examined, Y chromosome deletion was strongly and selectively associated with carcinomas, particularly adenocarcinomas, of the esophagus (P < .0001). These findings suggest that Y chromosome loss may be pathogenetically significant in these neoplasms. (C) 1993 Wiley-Liss, Inc.*
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页码:172 / 177
页数:6
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