NATURAL EVOLUTION OF CORONAVIRUS DEFECTIVE-INTERFERING RNA INVOLVES RNA RECOMBINATION

被引:19
作者
FURUYA, T
MACNAUGHTON, TB
LAMONICA, N
LAI, MMC
机构
[1] UNIV SO CALIF,SCH MED,HOWARD HUGHES MED INST,LOS ANGELES,CA 90033
[2] UNIV SO CALIF,SCH MED,DEPT MICROBIOL,LOS ANGELES,CA 90033
关键词
D O I
10.1006/viro.1993.1277
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Defective-interfering (DI) RNAs of RNA viruses, in general, are generated and continue to evolve in size during serial undiluted passages of viruses. This evolution was thought to occur by independent generation of DI RNAs during virus passages and subsequent selection of new DI RNAs under new cellular conditions. Here we demonstrate that recombination between the old DI RNA and the helper viral RNA can be one of the mechanisms for natural DI RNA evolution. A mouse hepatitis virus (MHV) DI RNA, DIssE RNA, was transcribed in vitro and transfected into a mouse cell line infected with a different MHV strain (A59), which is distinguishable from the original natural helper MHV (JHM). During subsequent serial undiluted passages of the harvested virus, several novel DI RNA species were generated, while the original DIssE RNA disappeared by passage 11. cDNA cloning and sequence analysis of one of these novel DI RNAs, designated DI-2, revealed that it is composed of four discontinuous regions of the genomic sequence and is different from the structure of the original DIssE RNA. Sequence comparison among DI-2, DIssE, and helper MHV-A59 RNAs showed that DI-2 sequence is similar to DIssE in the first and second regions, but similar to the helper A59 virus in the third and last regions. Thus, this DI RNA was generated by RNA recombination between the original DIssE RNA and the herper viral RNA. These results indicate that recombination between DI RNA and helper virus RNA can be involved in the natural evolution of DI RNAs. © 1993 Academic Press. All rights reserved.
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页码:408 / 413
页数:6
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